Advantages of TAPS 29915-38-6 application in nucleic acid capillary zone electrophoresis

In the fields of gene editing, disease diagnosis, and molecular biology research, precise separation and analysis of nucleic acids are the core steps to unlock the code of life. Capillary zone electrophoresis (CZE) has become an indispensable tool for nucleic acid research due to its high efficiency, speed, and trace technology advantages. However, challenges such as environmental stability, separation efficiency, and sample integrity during electrophoresis always constrain further technological breakthroughs. N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid (TAPS buffer), as a novel biological buffering agent, is revolutionizing nucleic acid electrophoresis due to its unique chemical properties.

1, Accurate pH regulation

The charge characteristics and migration behavior of nucleic acids are highly dependent on the environmental pH value. Traditional buffer systems are prone to pH drift due to electrolysis of water under high voltage electric fields, which can lead to poor reproducibility of DNA/RNA fragment separation and broadening of peak shapes. In the molecular structure of TAPS, the sulfonic acid group (- SO ∝⁻) forms a dynamic equilibrium with the amino group (- NH ₂), stabilizing its pKa value around 8.4, which is close to the physiological environment.

TAPS Powder

Compared to traditional buffering agents, the "steady state" characteristic of TAPS ensures precise migration of nucleic acid fragments in an electric field based on charge/mass ratio, avoiding separation deviations caused by pH fluctuations. Whether it is short stranded RNA or long stranded genomic DNA, TAPS can provide a consistent isolation environment, laying a reliable foundation for high-throughput sequencing and complex sample analysis.

2, Efficient thermal management

Improving the electric field strength is the key to enhancing separation efficiency, but the Joule heating generated by high voltage can easily lead to band broadening and even damage to nucleic acid structures. The molecular design of TAPS cleverly combines high ionic strength and strong hydration ability: the sulfonic acid group provides excellent conductivity, while the three hydroxymethyl groups (- CH ₂ OH) form a dense hydration layer through hydrogen bonding, accelerating heat diffusion.

3, Low background interference

Nucleic acid quantification relies on UV absorption or fluorescence detection, but most buffering agents have absorption peaks at the detection wavelength, forming background noise and limiting detection sensitivity. In addition, the low fluorescence background characteristics of TAPS are highly compatible with fluorescence labeling technology. In single-cell sequencing or CRISPR gene editing experiments, the TAPS buffer system can clearly distinguish individual base differences and even detect nucleic acid signals at the single-molecule level, providing strong technical support for precision medicine.

4, Mild biocompatibility

The integrity of nucleic acid samples is a prerequisite for the reliability of analysis results. Traditional strong alkaline buffering agents may cause DNA deprotonation or RNA hydrolysis, while the weak alkaline environment of TAPS is highly similar to the intracellular environment and does not contain nuclease activating factors. The hydroxymethyl group in its molecule forms hydrogen bonds with the nucleic acid phosphate backbone, further stabilizing the nucleic acid structure and reducing shear damage during electrophoresis. The mild nature of TAPS significantly improves sample recovery in the isolation of long stranded genomic DNA or sensitive circular RNA.

Product packaging

Hubei Xindesheng Material Technology, as a manufacturer of biological buffering agents, has rich experience in research and development and production. In addition to supplying common biological buffering agents such as TAPS, TRIS base, MOPS, CAPS in large quantities, Desheng can also provide customized services for small batches of biological buffering agents. While ensuring stable and excellent product performance, the price is affordable and we can provide good after-sales service. If you need it, you can click on the official website of Hubei Xindesheng for inquiries or contact me directly!