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TOOS 82692-93-1, a chromogenic substrate, can be used to determine the concentration of uric acid in human serum

Подробная информация о продукции

Место происхождения: Эжхоу, Китай

Фирменное наименование: DESHENG

Сертификация: ISO9001:2008

Номер модели: CAS 82692-93-1

Условия оплаты и доставки

Количество мин заказа: 100 мг

Цена: обсуждаемый

Упаковывая детали: Алюминиевая пленка или пластиковая бутылка запечатана

Время доставки: 1 ~ 3 дня после получения оплаты

Условия оплаты: T/TL/C PAYPAL

Поставка способности: 5t/месяц

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Спецификации
Выделить:

High purity reagent

,

Analytical Reagent

,

Highly water-soluble reagent

Цвет:
белый кристаллический порошок
Чистота:
≥99%
CAS №:
82692-93-1
Чистота:
99% мин
Молекулярный вес:
295.33
Молекулярная формула:
C12H18NNAO4S
Цвет:
белый кристаллический порошок
Чистота:
≥99%
CAS №:
82692-93-1
Чистота:
99% мин
Молекулярный вес:
295.33
Молекулярная формула:
C12H18NNAO4S
Описание
TOOS 82692-93-1, a chromogenic substrate, can be used to determine the concentration of uric acid in human serum

In the field of biochemical detection, accurate measurement of various indicators of the human body is of crucial significance for disease diagnosis, health assessment, and disease monitoring. Among them, uric acid, as a key product of purine metabolism in the body, can intuitively reflect the health status of the body at its concentration level. And the chromogenic substrate N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS reagent), with its unique properties, has become a powerful "assistant" for determining uric acid concentration in human serum.


Product Name

TOOS reagent

Product Appearance

White crystalline powder

CAS NO

82692-93-1

Standard Packaging

50g/bottle, plastic bottle

Molecular Weight

295.33

Molecular Formula

C12H18NNaO4S

Storage conditions

Room temperature, away from light and moisture

Manufacturer

Hubei Xindesheng


1, Uric Acid Measurement: A Key Window for Insight into Health


Uric acid plays an important role in the metabolic process of the human body. It originates from the metabolism of endogenous purine, where adenosine deaminase (ADA) catalyzes the deamination of adenine nucleoside to hypoxanthine nucleoside, which is then metabolized into urea through a series of enzymatic reactions. The normality of uric acid content is directly related to the health status of the body. When uric acid excretion is reduced or purine metabolism is excessively high, it may lead to hyperuricemia, which in turn can cause serious diseases such as gout, causing great pain to patients. On the contrary, insufficient or excessive production of uric acid can lead to hypouricemia, which can also have adverse effects on the body.


TOOS 82692-93-1, a chromogenic substrate, can be used to determine the concentration of uric acid in human serum 0

TOOS Powder


2, TOOS: An Outstanding Representative of the New Trinder's Reagent


TOOS belongs to the new type of Trinder's reagent, which has demonstrated extraordinary performance in biochemical testing. The enzymatic reaction between TOOS and hydrogen peroxide has extremely high sensitivity and specificity, making it an ideal choice for measuring the indicators that can generate hydrogen peroxide in biochemical detection. Among numerous application scenarios, using TOOS to measure uric acid concentration in human serum is one of its typical applications, providing reliable technical support for clinical diagnosis and scientific research.


3, TOOS enzymatic determination of uric acid: the scientific mystery of precise color development


Under the catalytic action of uricase, uric acid undergoes an oxidation reaction with oxygen and water, producing allantoin and hydrogen peroxide. This reaction is a crucial starting step in the entire measurement process. Subsequently, in the presence of peroxidase, the chromogenic substrate TOOS undergoes a coupling reaction with 4-aminoantipyrine (4-AAP), which is oxidized by the hydrogen peroxide generated in the previous step, resulting in the formation of red quinone imine compounds. The color depth of this substance is directly proportional to the amount of color products, which is closely related to the content of uric acid. By measuring the absorbance of the generated substance using a spectrophotometer, the concentration of uric acid in human serum can be accurately calculated based on the proportional relationship between absorbance and uric acid content.


In order to simplify the experimental operation and avoid the tedious process of repeatedly adding reagents, researchers cleverly divided the non reactive reagent combinations into two groups. Reagent 1 mainly includes biological buffer, peroxidase, and chromogenic substrate TOOS; Reagent 2 is mainly composed of uricase, 4-AAP, and sodium azide. During the experiment, simply mix these two sets of reagents in different proportions to easily complete the preparation work before the measurement.


TOOS 82692-93-1, a chromogenic substrate, can be used to determine the concentration of uric acid in human serum 1

Product packaging


In addition to TOOS enzyme photometry, there are also methods using chromogenic TOPS or TMB enzyme-linked immunosorbent assay to determine uric acid, which have similar principles. As a professional IVD raw material manufacturer, Hubei Xindesheng Material Technology Co., Ltd. can provide various chromogenic substrate such as TOOS, TOPS, MAOS, etc. If you need them, please feel free to contact me!

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